Calcium and zinc ions are essential for the -toxin to bind with lipids on the cell membrane and hydrolyze the substrate, respectively (Moreau et al., 1988)
Mishandling them during reconstitution can destroy the very molecules you need for your study
Oral glutathione has actual clinical trial support for modest skin brightening, while IV glutathione for cosmetic use does not no published human trial has established that injectable glutathione lightens skin, in India or anywhere else
J Biol Chem 275:3909039095 [DOI] [PubMed] [Google Scholar] Oldfield S, Braksator E, Rodriguez-Martin I, Bailey CP, Donaldson LF, Henderson G, Kelly E
Recommendations of the Immunization Practices Advisory Committee (ACIP)